Journal: Journal of Lipid Research

Article Title: Intrauterine oxygen milieu governs placental sphingolipid metabolism

doi: 10.1016/j.jlr.2025.100930

Figure Lengend Snippet: Expression of sphingolipid regulatory enzymes changes in early human placental development. A: representative immunoblots (left panel) and associated densitometry (right panel) for SMPD1 in human placental lysates collected between 5 and 15 weeks of gestation (no statistical differences, non-parametric Mann-Whitney test, 5–9 weeks: n = 7 samples; 10–15 weeks: n = 6 samples). B: representative immunoblots (left panel) and associated densitometry (right panel) for ACER2 in human placental lysates collected between 5 and 15 weeks of gestation (∗: P < 0.05, non-parametric Mann-Whitney test, 5–9 weeks: n = 4 samples; 10–15 weeks: n = 5 samples). C: Quantitative PCR for ASAH1 mRNA in placental lysates collected between 5 and 15 weeks of gestation (∗∗∗∗: P < 0.0001, unpaired Student’s t test, 5–9 weeks: n = 11 samples; 10–15 weeks: n = 11 samples). D: representative immunoblots (left panel) and associated densitometry (right panel) for ASAH1 in human placental lysates collected between 5 and 15 weeks of gestation (∗∗∗∗: P < 0.0001, unpaired Student’s t test, 5–9 weeks: n = 14 samples; 10–15 weeks: n = 13 samples). E: Quantitative PCR for SPHK1 mRNA in human placental lysates collected between 5 and 15 weeks of gestation (∗: P < 0.05, non-parametric Mann-Whitney test, 5–9 weeks: n = 10 samples; 10–15 weeks: n = 10 samples). F: representative immunoblots (left panel) and associated densitometry (right panel) for SPHK1 and SPHK2 in human placental lysates collected between 5 and 15 weeks of gestation (SPHK1: ∗∗: P < 0.01; SPHK2: no statistical significance, non-parametric Mann-Whitney test, 5–9 weeks: n = 14 samples; 10–15 weeks: n = 12 samples). w = weeks.

Article Snippet: The resulting cDNA were quantified by real-time PCR (CFX96 Real-Time System, Biorad) using PerfeCTa FastMix II from Quantabio (Beverly) and mouse specific TaqMan® (Assays-on-DemandTM) probes targeting 18s (Mm03928990_g1), Asah1 (Hs00602774_m1), Mm00480021_m1, Sphk1 (Hs00184211_m1, Mm00448841_g1), and Acer2 (Hs04996319_g1, Mm00519876_m1) from Applied Biosystems (ThermoFisher Scientific).

Techniques: Expressing, Western Blot, MANN-WHITNEY, Real-time Polymerase Chain Reaction

Journal: Journal of Lipid Research

Article Title: Intrauterine oxygen milieu governs placental sphingolipid metabolism

doi: 10.1016/j.jlr.2025.100930

Figure Lengend Snippet: Expression of sphingolipid regulatory enzymes is disrupted upon loss of placental Phd2 during murine pregnancy. A: Representative IHC staining for ASAH1 in WT and Phd2 −/− cKO placental sections at E14.5 (similar results were obtained in 3 separate WT and Phd2 −/− cKO placentae, respectively). D: decidua, L, labyrinthine, JZ junctional zone, scale bars = 500 μm. B: representative immunoblot (top panel) and associated densitometry (bottom panel) for ASAH1 in WT and Phd2 −/− cKO murine placental samples collected at E14.5 (∗∗∗: P < 0.001, non-parametric Mann-Whitney test; n = 4 WT litters, 10 placentae, and n = 4 Phd2 −/− cKO litters, 10 placentae). C: quantitative PCR for Acer2 mRNA in WT and Phd2 −/− cKO murine placental samples collected at E14.5 (no statistical differences, unpaired Student’s t test; n = 4 WT litters, 12 placentae, and n = 5 Phd2 −/− cKO litters, 13 placentae). D: representative immunoblot (left panel) and associated densitometry (right panel) for ACER2 in WT and Phd2 −/− cKO murine placental samples collected at E14.5 (no statistical differences, non-parametric Mann-Whitney test; n = 4 WT litters, 4 placentae, and n = 4 Phd2 −/− cKO litters, 5 placentae). E: representative immunoblots (left panel) and associated densitometry (right panel) for SPHK1 and SPHK2 in WT and Phd2 −/− cKO placental samples collected at E17.5 (∗: P < 0.05, unpaired Student’s t test; n = 4 WT litters, 8 placentae, and n = 4 Phd2 −/− cKO litters, 8 placentae). ACTB was used as loading control. ns = nonsignificant.

Article Snippet: The resulting cDNA were quantified by real-time PCR (CFX96 Real-Time System, Biorad) using PerfeCTa FastMix II from Quantabio (Beverly) and mouse specific TaqMan® (Assays-on-DemandTM) probes targeting 18s (Mm03928990_g1), Asah1 (Hs00602774_m1), Mm00480021_m1, Sphk1 (Hs00184211_m1, Mm00448841_g1), and Acer2 (Hs04996319_g1, Mm00519876_m1) from Applied Biosystems (ThermoFisher Scientific).

Techniques: Expressing, Immunohistochemistry, Western Blot, MANN-WHITNEY, Real-time Polymerase Chain Reaction, Control